Research Article | Published: 31 March 2025

Investigation of plant hormone effects on the growth and development in vitro propagation of Rosa Indica plant: A study

Bhawana  Goswami, Shahana Jabi, Hem Chandra Pant, Abhishek Bhardwaj, Amandeep Singh, Ashok Kumar, Shanti Rauthan and Amita Pandey

Journal of Non-Timber Forest Products | Volume: 32 | Issue: 1 | Page No. 10-16 | 2025
DOI: https://doi.org/10.54207/bsmps2000-2025-8899U0 | Cite this article

Abstract

Roses are valued for their ornamental, therapeutic, and commercial importance; their petals possess antibacterial properties, while the leaves are rich in vitamins and essential nutrients. Present work aimed to identify the optimal growth regulators and basal medium for the propagation of Rosa indica. The study revealed that an MS medium supplemented with 2.0 mg L-¹ BAP and 1.5 mg L-¹ IAA was optimal for shoot initiation, with shoot formation observed within two weeks from nodal explants. Maximum shoot multiplication was achieved using a combination of 2.5 mg L-¹ BAP and 1.5 mg L-¹ kinetin under optimized PGR concentrations. For rooting, MS medium fortified with 2.0 mg L-¹ IBA in combination with 1.0 mg L-¹ BAP proved most effective. Subsequently, well-rooted plantlets were acclimatized under ex vitro conditions by washing the roots with distilled water and transferring them to small cups containing a sand:soil:manure mixture (1:1:1), resulting in vigorous growth and a survival rate of 65%.

Keywords

BAP, Kinetin, MS medium, Nodal explants, Rose, Soil

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How to cite

Goswami, B., Jabi, S., Pant, H.C., Bhardwaj, A., Singh, A., Kumar, A., Rauthan, S. and Pandey, A., 2025. Investigation of plant hormone effects on the growth and development in vitro propagation of Rosa Indica plant: A study. Journal of Non-Timber Forest Products, 32(1), pp.10-16. https://doi.org/10.54207/bsmps2000-2025-8899U0

Publication History

Manuscript Received on 03 March 2025

Manuscript Revised on 24 March 2025

Manuscript Accepted on 26 March 2025

Manuscript Published on 31 March 2025

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