Research Article | Published: 01 March 1996

Organogenesis and Oil Production in Neem Seed Callus

J. P. Rier, Jr. and Silas Obasi

Indian Journal of Forestry | Volume: 19 | Issue: 1 | Page No. 1-5 | 1996
DOI: https://doi.org/10.54207/bsmps1000-1996-M89E42 | Cite this article

Abstract

Callus initiated from germinating seeds of Azadirachta indica A. Juss. was maintained on a modified Murashige and Skoog medium. Organogenesis occurred in tissues subcultured onto medium supplemented with various growth substances. Undifferentiated callus tissues and seeds without testa were extracted with hexane. The extract was treated with 500 µl each of methanol and diazomethane, then standardized with 100 µl of squalene. An analysis by mass spectrometry indicated eight fatty acids in callus tissues; myristic, palmitic, stearic, oleic, linoleic, arachidic and behemic. Four fatty acids; palmitic, stearic, oleic and linoleic were found in the seed extract.

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How to cite

Rier, Jr., J.P. and Obasi, S., 1996. Organogenesis and Oil Production in Neem Seed Callus. Indian Journal of Forestry, 19(1), pp.1-5. https://doi.org/10.54207/bsmps1000-1996-M89E42

Publication History

Manuscript Published on 01 March 1996

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