Research Article | Published: 01 June 1998

Bacterial contamination in micropropagation of matured Sandal (Santalum album L.) plus tree

Manojkumar M. Reddy and Sukanya Subramanian

Indian Journal of Forestry | Volume: 21 | Issue: 2 | Page No. 108-110 | 1998
DOI: https://doi.org/10.54207/bsmps1000-1998-0475D2 | Cite this article

Abstract

The micropropagation of 65- year old sandal (Santalum album L.) plus tree was unsuccessful due to consistent occurrence of both fungal and bacterial contamination in all cultures. The fungal contamination was eliminated by the use of Bavistin, a systemic fungicide, in the sterilization procedure. Bacterial isolate from the contaminated cul­tures was found to contain only one type of bacterium, Gram-negative Pseudomonas. The antibiotic sensitivity disc test revealed Tetracycline as the most effective antibiotic followed by Rifampicin and a homaeopathic medicine, Calendula against Pseudomonas while Chloramphenicol, Ampicillin, Strepto-penicillin, etc., were ineffective. Thus, a careful selection of antibiotic can eliminate the contaminants and reduce huge losses in tissue culture of woody perennials.

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How to cite

Reddy, M.M. and Subramanian, S., 1998. Bacterial contamination in micropropagation of matured Sandal (Santalum album L.) plus tree. Indian Journal of Forestry, 21(2), pp.108-110. https://doi.org/10.54207/bsmps1000-1998-0475D2

Publication History

Manuscript Published on 01 June 1998

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